Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	706,132	T→C	G589G (GGT→GGC)	nagE →	fused N‑acetyl glucosamine specific PTS enzyme IICBA components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	706,132	0	T	C	100.0%	60.3 / NA	20	G589G (GGT→GGC)	nagE	fused N‑acetyl glucosamine specific PTS enzyme IICBA components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/0);  total (20/0)

TCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGATT  >  W3110S.gb/706117‑706178                |                                               tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCa                            >  1:1484523/1‑36 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:2072724/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:794780/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:675513/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:460836/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:426545/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:374343/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:279060/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:2708247/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:2580821/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:2563920/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1309600/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1920745/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1874801/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1754432/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1733671/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1630184/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1408355/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAtt  >  1:1388177/1‑62 (MQ=255) tCTGGTGGAAGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGAt   >  1:1912141/1‑61 (MQ=255)                |                                               TCTGGTGGAAGAGGGTGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGATT  >  W3110S.gb/706117‑706178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑