Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	746,776	G→A	V140V (GTG→GTA)	nei →	endonuclease VIII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	746,776	0	G	A	83.1%	167.6 / 24.4	77	V140V (GTG→GTA)	nei	endonuclease VIII
Reads supporting (aligned to +/- strand):  ref base G (0/13);  new base A (64/0);  total (64/13)
Fisher's exact test for biased strand distribution p-value = 5.44e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTA  >  W3110S.gb/746754‑746827                       |                                                    cGAATCTGACGCCGGAGGTGGTAAAGGATCGATTATTGTCGCCGCGCTTTCGTa                      >  1:2284243/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTcg                                   >  1:2583841/1‑41 (MQ=38) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt                       >  1:1027565/1‑53 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt                       >  1:1201929/1‑53 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt                       >  1:1474505/1‑53 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt                       >  1:1759269/1‑53 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2392078/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2749300/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2691015/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:255019/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2536636/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2534779/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2507642/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:387786/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2367107/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2320981/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2287155/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2274287/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1018640/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:215862/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:555866/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:993495/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:858576/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:850466/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:7630/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:739691/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:675410/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:658259/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:618256/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2898343/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:548803/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:505014/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:498459/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:425353/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:422087/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:218222/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:360710/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1370777/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1665731/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1595637/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1576161/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1528948/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:151533/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1490190/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1436672/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1379686/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1577985/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1347056/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1338258/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1321508/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1217147/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1073188/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1070046/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1027349/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1867435/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2110173/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:2106980/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:195491/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1885007/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:187130/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1765528/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1714964/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa                      >  1:1686981/1‑54 (MQ=255) cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGAGCTTTCGTa                      >  1:1207253/1‑54 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:1725238/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:1797974/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:26245/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:1561632/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:2324225/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:671675/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:2581452/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:2156996/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:821933/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:1972550/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:874918/62‑1 (MQ=255)             cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:2735945/62‑1 (MQ=255)              ggAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa  <  1:2440522/61‑1 (MQ=255)                       |                                                    CGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTA  >  W3110S.gb/746754‑746827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑