Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	901,654	A→G	N101N (AAT→AAC)	artM ←	arginine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	901,654	0	A	G	100.0%	187.9 / NA	55	N101N (AAT→AAC)	artM	arginine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/0);  total (55/0)

GCACTATTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAG  >  W3110S.gb/901649‑901710      |                                                         gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGacaaca            >  1:1116499/1‑52 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCcaca   >  1:1737504/1‑61 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCTACAg  >  1:104527/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2548650/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:996913/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2597812/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2620358/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2648037/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2710077/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2724538/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2817024/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2853499/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2857194/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2859849/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:327914/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:376317/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:569023/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:579884/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:593509/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:596883/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:713287/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:815342/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:820706/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:827484/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:86908/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:931095/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:937286/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1043248/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1749489/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:106246/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:11133/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1197495/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1231433/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1289487/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1306599/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1441549/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1481903/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1700593/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1731753/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2485130/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:175608/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1768371/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:1944792/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2034932/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2041435/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2064040/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2099691/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2103791/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2177129/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2232295/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2244431/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:2257677/1‑62 (MQ=255) gcACTGTTCAGCGACAACGCAATCAGCGAACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:776355/1‑62 (MQ=255) gcACTGTTCAACGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:840750/1‑62 (MQ=255) acACTGTTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAg  >  1:59287/2‑62 (MQ=255)      |                                                         GCACTATTCAGCGACAACGCAATCAGCGCACATAACCACGGTTCTGACAACAAATGCCACAG  >  W3110S.gb/901649‑901710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑