BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	919,423	A→T	R40R (CGT→CGA)	ybjX ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	919,423	0	A	T	100.0%	166.0 / NA	50	R40R (CGT→CGA)	ybjX	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (50/0); total (50/0)

GCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAT  >  W3110S.gb/919362‑919468

gcatcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:218190/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1193885/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:2050920/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:2187839/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1983378/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1917934/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1834251/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1666205/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:2711593/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:2841280/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:154154/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:343742/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:630990/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:454321/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:453352/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCa                                               >  1:1356980/1‑62 (MQ=255)

gcagcaAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACACGGCCGACACTGTCCa                                               >  1:2465636/1‑62 (MQ=255)

                                             ccGGCCGACATTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2052069/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGTCAGgctg                 >  1:2845691/1‑47 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGAc                       >  1:1972638/1‑41 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAgg                    >  1:2601205/1‑44 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAgg                    >  1:465991/1‑44 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTga             >  1:2380024/1‑51 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAg    >  1:2761533/1‑60 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:2436498/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:382195/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:2418959/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:345358/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:2392841/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:225471/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:955954/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:2078987/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:1581767/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:144083/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:1599180/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:1655339/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:1944849/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGa   >  1:1930806/1‑61 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1454771/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2712500/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1399985/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1354498/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:396360/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1291508/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1252473/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1227860/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:51661/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:533205/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1221467/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:883118/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:89130/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:121908/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1577793/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2801613/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2093257/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2693092/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2486029/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1777554/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1810146/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1892319/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2337494/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2279475/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:1983757/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:22374/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2036969/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:2183575/1‑62 (MQ=255)

                                             ccGGCCGACACTGTCCTCGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAt  >  1:214469/1‑62 (MQ=255)

GCAGCAAAAATTTCTGGCGAAAACTACGGCGATGCCAGAATTTACCCGGCCGACACTGTCCACGTGCCAGACTAAGAAAAAGTGACAGGCTGCTGAGAGATTCAGAT  >  W3110S.gb/919362‑919468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`