Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	930,215	G→A	S390S (AGC→AGT)	cydD ←	fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	930,215	0	G	A	98.2%	172.9 / ‑4.5	54	S390S (AGC→AGT)	cydD	fused cysteine transporter subunits and membrane component and ATP‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (53/0);  total (54/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGA  >  W3110S.gb/930168‑930236                                                |                      aGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAAcc         >  1:1723799/1‑62 (MQ=255)        ccTGATATGAGAGAAAACCATAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1813441/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAACGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:109115/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTgacc  >  1:2752302/1‑59 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGc    >  1:424091/1‑60 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2684070/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:97848/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2810473/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2811759/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2812834/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:304641/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:318596/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:383303/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:438662/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:442210/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:50294/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:570058/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:590851/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:648284/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:650130/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:67073/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:784073/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:784654/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:789984/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:864163/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:912089/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:915221/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:976806/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1025835/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2274420/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1130069/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1146536/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1291526/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1340538/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1360290/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1625706/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:167132/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1803302/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:1941534/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2168417/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:217402/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2268401/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2678276/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2351163/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2379587/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2410965/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2453859/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2459115/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:249625/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2516532/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2584505/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2648639/1‑61 (MQ=255)        ccTGATATGAGAGAAAACCAGAAAGCGAGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2654172/1‑61 (MQ=255)        catGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAACTTTTACCTGAACCGCTGCg   >  1:2657476/3‑61 (MQ=255)                                                |                      AGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGA  >  W3110S.gb/930168‑930236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑