Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	940,801	T→C	L318L (TTG→CTG)	serS →	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	940,801	0	T	C	100.0%	155.1 / NA	46	L318L (TTG→CTG)	serS	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/0);  total (46/0)

CCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGG  >  W3110S.gb/940741‑940856                                                             |                                                        ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:312436/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2398651/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2335870/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2475593/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2276629/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2483867/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2601654/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2040552/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:1965954/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:1877383/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2668883/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:2699971/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:348640/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:384665/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:49129/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:150655/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:689406/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:699965/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:1337194/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:884974/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:1259214/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:1158960/1‑62 (MQ=255) ccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt                                                        >  1:976087/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCTTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:1659274/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTAc                    >  1:2558866/1‑44 (MQ=38)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGAGATGGGCTTTg   >  1:998409/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACa            >  1:977552/1‑52 (MQ=38)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATgg         >  1:2868410/1‑55 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2703023/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:617099/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1512839/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:489047/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:459206/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:417123/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:40862/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1544439/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1483712/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1428494/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2824409/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:815/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1283109/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1732300/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:91294/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:1871038/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:98199/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2372139/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2533762/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2529930/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2490584/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2486206/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2247091/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2281469/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg  >  1:2461784/1‑62 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:104729/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:1107234/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:727674/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:687808/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:644352/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:605693/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:1697165/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:1720616/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2923194/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2813315/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2716126/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2672862/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2088989/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2579472/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:25669/1‑61 (MQ=255)                                                       cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg   >  1:2232178/1‑61 (MQ=255)                                                             |                                                        CCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGG  >  W3110S.gb/940741‑940856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑