| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 52,730 | C→T | V229V (GTG→GTA) | pdxA ← | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 52,730 | 0 | C | T | 92.0% | 66.8 / ‑1.6 | 25 | V229V (GTG→GTA) | pdxA | 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (23/0); total (23/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCCCGCAGCTCATTGAGCACCG > W3110S.gb/52672‑52734 | gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2434887/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:990296/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:94877/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:88938/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:765867/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:608935/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:342663/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2904094/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2836884/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2765540/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2749664/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2581486/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2530776/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1188076/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:238426/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:238048/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:2328305/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:183977/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1553416/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1483522/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1461900/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1342273/1‑61 (MQ=255)gggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGTTCCCGCAGCTCGTTGAGTAc > 1:1217050/1‑61 (MQ=255) ggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCCCGCAGCTCATTGAGCACCg < 1:1784733/62‑1 (MQ=255) ggTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCCCGCAGCTCATTGAGCACCg < 1:1752955/62‑1 (MQ=255) | GGGTATCGGCAGGCAGCGGCCCGTTGAGTTTCATCCCCTGCGCCCGCAGCTCATTGAGCACCG > W3110S.gb/52672‑52734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |