Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	940,839	T→C	T330T (ACT→ACC)	serS →	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	940,839	0	T	C	100.0%	238.0 / NA	71	T330T (ACT→ACC)	serS	seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/26);  total (45/26)

CTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAAC  >  W3110S.gb/940795‑940868                                             |                              cTGCAGCTGCTGGGCCTGCCGTACCTTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:1659274/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGAGATGGGCTTTg               >  1:998409/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACa                        >  1:977552/1‑52 (MQ=38) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATgg                     >  1:2868410/1‑55 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2703023/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2247091/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2281469/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2372139/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2461784/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2486206/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2490584/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:98199/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2529930/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:91294/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2824409/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:2533762/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:40862/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:417123/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:459206/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:489047/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:617099/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:815/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1428494/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1283109/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1871038/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1483712/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1732300/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1544439/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTgg              >  1:1512839/1‑62 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2088989/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:605693/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:644352/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:687808/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2923194/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:727674/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2813315/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2716126/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2672862/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2579472/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:25669/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:104729/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:1697165/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:2232178/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:1720616/1‑61 (MQ=255) cTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTg               >  1:1107234/1‑61 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1306959/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1092669/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2081293/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1095029/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:981228/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:978606/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:74996/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1231341/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:896116/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1196602/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1308523/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:453635/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1354158/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:395084/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:336471/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:320660/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2847729/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1502086/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2681262/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2577180/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2448843/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:176561/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2323734/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2322080/60‑1 (MQ=255)               ccTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:1882879/60‑1 (MQ=255)                cTGCCGTACCGTAAAATCATCCTTTGTACCGGCGACATGGGCTTTGGCGCTTGCAAAAc  <  1:2850215/59‑1 (MQ=255)                                             |                              CTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAAAC  >  W3110S.gb/940795‑940868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑