Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	956,326	T→C	P243P (CCA→CCG)	ycaO ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	956,326	0	T	C	100.0%	100.9 / NA	31	P243P (CCA→CCG)	ycaO	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/31);  total (0/31)

ACCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATTGGGAAACC  >  W3110S.gb/956273‑956334                                                      |         aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTTATAAGCGAAGATCGGGAAAcc  <  1:695296/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1300230/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:840942/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:639425/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:564047/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:563334/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:487976/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:408985/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2826972/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2789299/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2730760/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2627144/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2626448/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2625285/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:259063/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2508251/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2503320/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2477461/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2473474/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2124933/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:210020/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:2025889/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1881533/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1846980/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1731044/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1591676/62‑1 (MQ=255) aCCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1152682/62‑1 (MQ=255)  ccACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1742071/61‑1 (MQ=255)  ccACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:888654/61‑1 (MQ=255)                  aTACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCGGGAAAcc  <  1:1888055/45‑1 (MQ=255)                  aTACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATCAGGAAAcc  <  1:1354006/45‑1 (MQ=38)                                                      |         ACCACGCAAATCACCGGATACTGGCCGCCAAGCGAACCGTCATAAGCGAAGATTGGGAAACC  >  W3110S.gb/956273‑956334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑