Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	962,878	T→C	F154F (TTT→TTC)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	962,878	0	T	C	100.0%	82.4 / NA	25	F154F (TTT→TTC)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/0);  total (25/0)

GCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCT  >  W3110S.gb/962862‑962923                 |                                              gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2340252/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:73609/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:61622/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:521884/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:292086/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2787974/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2691005/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2686697/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2675201/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2604872/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2575212/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2537290/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2363795/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:1179781/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2248193/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2243127/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2210010/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:209495/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2041227/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:2006370/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:1936810/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:1936752/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:1712833/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtct  >  1:1251349/1‑62 (MQ=255) gCAAAGAGCTTGAATTCAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTtc   >  1:1772864/1‑61 (MQ=255)                 |                                              GCAAAGAGCTTGAATTTAAAGTAATCAAGCTGGATCAGAAGCGCAACAACGTTGTTGTTTCT  >  W3110S.gb/962862‑962923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑