| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 974,852 | C→A | L38M (CTG→ATG) | mukF → | Involved in chromosome partioning, Ca2+ binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 974,852 | 0 | C | A | 100.0% | 20.3 / NA | 8 | L38M (CTG→ATG) | mukF | Involved in chromosome partioning, Ca2+ binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/8); total (0/8) | |||||||||||
GACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGT > W3110S.gb/974823‑974884 | caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:1277125/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:1515385/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:1524449/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:1915070/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:2321937/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:490758/61‑1 (MQ=255)caCTCTCTTTTCTGCTGGCGGTTGCCACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:890050/61‑1 (MQ=255) cACGATGAACGGCGAGCGTCTGGATGGTGAGATGAGt < 1:2845768/37‑1 (MQ=255) | GACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGT > W3110S.gb/974823‑974884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |