Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,578,794	2 bp→GC	coding (539‑540/2373 nt)	yddB ←	predicted porin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,578,794	0	C	G	100.0%	139.7 / NA	42	K180S (AAG→AGC)	yddB	predicted porin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (42/0);  total (42/0)
*	W3110S.gb	1,578,795	0	T	C	100.0%	140.2 / NA	42	K180S (AAG→AGC)	yddB	predicted porin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/0);  total (42/0)

TGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGC  >  W3110S.gb/1578747‑1578808                                                ||              tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2852913/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1018681/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2279275/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2284391/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2358330/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2368706/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2489824/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2589544/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:266411/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:275165/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2837041/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2158716/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:413436/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:581843/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:65581/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:666845/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:673975/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:723755/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:732539/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:79110/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:979709/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1412793/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1028721/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1042278/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1064463/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1154387/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1188643/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1242395/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1358892/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1360483/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1360792/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1395300/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2111233/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:146850/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1686598/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1732188/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1739649/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:1755249/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2010922/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2067875/1‑62 (MQ=255) tGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAGTTTCACGCTACTATCATCAGc  >  1:193740/1‑62 (MQ=255) tGCGATGTTAACCAGTACGAACGCGTAGTGCGATAACCCAGTTTCACGCTGCTATCATCAGc  >  1:2198341/1‑62 (MQ=255)                                                ||              TGCGATGTTAACCAGTCCGAACGCGTAGTGCGATAACCCAATTTCACCTTGCTATCATCAGC  >  W3110S.gb/1578747‑1578808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑