Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,062,649	Δ1 bp	coding (429/600 nt)	torD →	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,062,646	0	G	.	100.0%	98.1 / NA	24	coding (426/600 nt)	torD	chaperone involved in maturation of TorA subunit of trimethylamine N‑oxide reductase system I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/0);  total (24/0)

GCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCG  >  W3110S.gb/1062616‑1062663                               |                  gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTgcg    >  1:383627/1‑45 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:323830/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:926061/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:810639/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:789539/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:765970/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:663779/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:623382/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:4721/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:466617/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:428646/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:389326/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1003522/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:287370/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1561632/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1373513/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1317785/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1314907/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1254921/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1235350/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1153456/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1133899/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1077533/1‑47 (MQ=255) gCTCAGCCATCTGCATTTTTCGCTGGGAGA‑GGGACCGTTCCTGcgcg  >  1:1015484/1‑47 (MQ=255)                               |                  GCTCAGCCATCTGCATTTTTCGCTGGGAGAGGGGACCGTTCCTGCGCG  >  W3110S.gb/1062616‑1062663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑