| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 366,370 | Δ1 bp | coding (365/1083 nt) | lacI ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 366,370 | 0 | A | . | 92.0% | 86.2 / 5.1 | 25 | coding (365/1083 nt) | lacI | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base . (0/23); total (2/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
AGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGAT > W3110S.gb/366330‑366397 | aGCAATGGCATCCTGGTCATCCATCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1195666/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:797847/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1154175/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:786859/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:725407/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:541957/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:412569/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:348769/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:307339/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:266229/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:189170/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1637967/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1632483/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:156247/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1549077/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1524971/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1467668/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1350877/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1332521/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1320921/62‑1 (MQ=255)aGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1285605/62‑1 (MQ=255) ggCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGAt > 1:1428276/1‑62 (MQ=255) ggCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGAt > 1:601110/1‑62 (MQ=255) ccTGGTCATCCAGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:289009/51‑1 (MQ=255) aGCGGATAGTTAATGATC‑GCCCACTGACGCGTTGCGCgag < 1:1270620/40‑1 (MQ=39) | AGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGAT > W3110S.gb/366330‑366397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |