Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,798,022	+A	coding (1637/2388 nt)	pheT ←	phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,798,019	1	.	A	100.0%	40.1 / NA	16	S547F (TCT→TTT)	pheT	phenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/16);  total (0/16)

CCACGGTTGCCAGCAGGCCAGTCCACAGAG‑AAAGACGCATTGCTGACATTTCAACAGAGAT  >  W3110S.gb/1797990‑1798050                               |                                ccacGGTTGCCAGCAGTCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:1349945/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:105314/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:1136351/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:1165761/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:1207661/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:134781/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:1493794/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:164084/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:468878/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:506620/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:521973/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:669220/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:794327/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:856793/62‑1 (MQ=255) ccacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:891920/62‑1 (MQ=255)  cacGGTTGCCAGCAGGCCAGTCCACAGAGAAAAGACGCATTGCTGACATTTCAACAGAGAt  <  1:121149/61‑1 (MQ=255)                               |                                CCACGGTTGCCAGCAGGCCAGTCCACAGAG‑AAAGACGCATTGCTGACATTTCAACAGAGAT  >  W3110S.gb/1797990‑1798050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑