Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,900,563	(A)6→7	coding (12/1479 nt)	ygcE →	predicted kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,900,557	1	.	A	100.0%	94.7 / NA	34	S2S (TCG→TCA)	ygcE	predicted kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/34);  total (0/34)

AATCATGTCG‑AAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTG  >  W3110S.gb/2900548‑2900608           |                                                    aaTCATGTCGAAAAAAATACATCATAGTGATTGATGGCGGAAGTCAGAGCACAAACgtggtg  <  1:314309/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgttgtg  <  1:377738/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:514235/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:363926/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:364141/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:372034/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:437526/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:454441/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:473134/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:353811/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:53908/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:577248/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:607412/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:644609/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:78929/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:850095/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:996276/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1462672/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1078451/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1110798/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1213791/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1219000/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1253014/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1258544/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1443412/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1050704/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1463116/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1510560/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1535525/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1552967/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:1590564/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg  <  1:352179/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgaggtg  <  1:453121/62‑1 (MQ=255) aaTCATGTCGAAAAAAATACATCATAGGGATTGATGGCGGAAGTCACAGCACAAAAgtggtg  <  1:1460541/62‑1 (MQ=255)           |                                                    AATCATGTCG‑AAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTG  >  W3110S.gb/2900548‑2900608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑