| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,819,124 | +T | intergenic (‑135/‑123) | gmk ← / → ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,819,121 | 1 | . | T | 100.0% | 14.2 / NA | 7 | intergenic (‑132/‑126) | gmk/ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (7/0); total (7/0) | |||||||||||
CTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCA‑TTTGCCTGTCA > W3110S.gb/3819073‑3819132 | cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTc > 1:1607409/1‑60 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1170049/1‑61 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:130207/1‑61 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1337788/1‑61 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:1460092/1‑61 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:713507/1‑61 (MQ=255)cTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCATTTTGCCTGTCa > 1:798501/1‑61 (MQ=255) | CTCACACTTTTTGCGCAAAGTTGTTGCGTTTTGCGTAGCTGCTTCCGCA‑TTTGCCTGTCA > W3110S.gb/3819073‑3819132 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |