Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,176,073	+G	coding (424/1164 nt)	gspL ←	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,176,070	1	.	G	97.1%	91.5 / ‑3.3	34	coding (427/1164 nt)	gspL	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (33/0);  total (34/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GTCCAGCTATCTACTTCCCA‑GGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGA  >  W3110S.gb/4176051‑4176111                     |                                           gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCa                           >  1:1005382/1‑37 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCAc       >  1:1142114/1‑57 (MQ=39) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTg   >  1:1159549/1‑61 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTg   >  1:318585/1‑61 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTg   >  1:391472/1‑61 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTg   >  1:796748/1‑61 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:714427/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:286708/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:302520/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:560009/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:703708/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:709159/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:253106/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:799467/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:83977/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:905015/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:931571/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:976808/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:272017/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:250178/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1592014/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1524522/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1493625/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1457160/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1378026/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1376526/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1324183/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1261507/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:123750/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1188939/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:109356/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:1016856/1‑62 (MQ=255) gTCCAGCTATCTACTTCCCAGGGG‑AAGATAGCAGCCAGCTgg                      >  1:1356493/1‑42 (MQ=255) gTCCAGCTATCTACTTCCCA‑GGGTAAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGa  >  1:975201/1‑62 (MQ=255)                     |                                           GTCCAGCTATCTACTTCCCA‑GGG‑AAGATAGCAGCCATCTGGCAAAACCCGCGTCACGTTGA  >  W3110S.gb/4176051‑4176111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑