Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	W3110S.gb	4117595	4118069	475	35 [0]	[0] 86	[yrfD]	[yrfD]

CTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAACGC  >  W3110S.gb/4117533‑4117594                                                              | cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:709985/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:466579/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:516437/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:548739/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:571838/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:626165/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:644953/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:662692/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:700121/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:463992/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:749000/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:818061/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:884892/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:888858/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:939847/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:940106/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:997169/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:146662/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1153528/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1168253/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1186455/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1254720/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1321619/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1385704/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1412547/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1457788/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1067587/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1533261/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:1537703/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:189319/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:372678/62‑1 (MQ=255) cTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:37407/62‑1 (MQ=255)  tttACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:241131/61‑1 (MQ=255)        aaCTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:369246/55‑1 (MQ=255)            tCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAAcgc  <  1:694508/51‑1 (MQ=255)                                                              | CTTTACGAACTTCACTGGAAATTTCCCATTTAGTTTCATTTGGGCAGTTTATAAACAAACGC  >  W3110S.gb/4117533‑4117594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑