| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 215,677 | 0 | G | C | 74.2% | 473.6 / 180.0 | 283 | A137P (GCG→CCG) | nlpE | lipoprotein involved with copper homeostasis and adhesion |
| Reads supporting (aligned to +/- strand): ref base G (3/70); new base C (210/0); total (213/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 283 total aligned reads displayed.
CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATT > W3110S.gb/215632‑215728 | cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACTCGCTGGAACCGGCACATTCCAGctt > 1:904686/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct > 1:58297/1‑59 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct > 1:906772/1‑59 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgct > 1:1205644/1‑59 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAgc > 1:1046648/1‑59 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:287955/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:500843/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:489253/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:464351/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:423379/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:412566/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:399360/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:392412/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:369427/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:348492/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:327910/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1649645/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:279435/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:274504/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:261715/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:252215/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:243502/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:230599/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:199437/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:180047/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1022014/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:766077/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:985838/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:977318/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:973153/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:956026/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:948206/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:934289/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:8681/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:837399/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:80744/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:802150/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:507637/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:744056/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:736727/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:705661/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:658574/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:644323/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:637728/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:592962/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:56892/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:549230/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1160718/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1364213/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1340185/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1325674/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1312923/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1309596/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1288869/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1275521/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1237804/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1213166/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1624035/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1155384/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1134005/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:111918/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1101572/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1088967/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:108280/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1034823/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1010231/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1573211/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1499578/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1478909/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1522931/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1557338/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1454346/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1445149/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1407348/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:159083/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1404590/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1384327/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:161317/1‑62 (MQ=255)cGTGAAGGTAATCCGATTGAATCGCAGTTCAACTACACGCTGGAACCGGCACAATCCAGctt > 1:1375075/1‑62 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1632790/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:21754/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1149761/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1543090/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1197610/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1027306/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1227158/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:676712/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:306884/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:622960/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:141225/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:525249/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1329166/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1373501/62‑1 (MQ=255) tCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:45174/62‑1 (MQ=255) ccGATTGAATCTCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1177785/61‑1 (MQ=255) ccGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1264289/61‑1 (MQ=255) ccGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:237081/61‑1 (MQ=255) cAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:450188/49‑1 (MQ=255) tataCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGc < 1:1252969/40‑1 (MQ=255) aCGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTAtt > 1:747548/1‑61 (MQ=255) | CGTGAAGGCAATCCGATTGAATCGCAGTTCAACTATACGCTGGAAGCGGCACAATCCAGTTTACCTATGACGCCGATGACCCTGCGGGGCATGTATT > W3110S.gb/215632‑215728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |