Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb625,7180CT67.8% 212.6 / 143.0 214F142F (TTC→TTT) entE2,3‑dihydroxybenzoate‑AMP ligase component of enterobactin synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base C (0/69);  new base T (145/0);  total (145/69)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.21e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

GTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAG  >  W3110S.gb/625688‑625772
                              |                                                      
ttttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1637654/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCgtggtga                         >  1:1096476/2‑61 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCgtggtg                          >  1:1540566/2‑61 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCgtggtg                          >  1:1135862/2‑61 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCgtggt                           >  1:860226/2‑60 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:562595/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:591238/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:542599/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:539923/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:51871/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:509291/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:498434/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:446958/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:438062/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:426332/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:388033/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:377673/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:305877/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:237851/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1005148/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:206830/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:201033/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:189853/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:177507/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:794328/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:99274/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:986097/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:955871/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:949352/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:943596/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:934219/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:895748/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:85693/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:818242/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:573928/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:769129/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:74206/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:729058/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:697650/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:684733/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:652466/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:636087/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:610743/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:231166/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1277555/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1440401/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1429644/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1424472/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1419965/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1401295/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1381204/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1332642/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1324240/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1291631/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1605835/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1259995/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1244780/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1224163/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1196575/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1166539/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1130551/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1062402/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1055867/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1502933/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1455157/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1601920/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1496594/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1593129/2‑62 (MQ=255)
atttAGCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCGATTCGCGTGGTGc                         >  1:1553488/2‑62 (MQ=255)
                        tACTTTCGTCACAGAACATTTCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:397864/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGat  <  1:1179449/61‑2 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1146385/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1534190/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:217777/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:782521/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:793553/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1184485/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:267911/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:86459/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1128189/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1081446/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1649811/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1036817/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1012843/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:250465/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:676536/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1311315/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:310186/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1328158/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:320951/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:135209/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:341715/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:365917/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:535448/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:520919/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:141363/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1481397/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:1461473/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:417776/61‑1 (MQ=255)
                        tACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAg  <  1:432476/61‑1 (MQ=255)
                              |                                                      
GTTTAGCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCTCAACGACAGCGGTGAG  >  W3110S.gb/625688‑625772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑