| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 756,655 | 0 | C | T | 68.1% | 207.7 / 136.8 | 208 | L109L (CTC→CTT) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/66); new base T (141/0); total (142/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 212 total aligned reads displayed.
CTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCG > W3110S.gb/756617‑756710 | ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCaa > 1:1356172/1‑60 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:224343/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:24184/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:210487/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:204014/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:194676/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:181474/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1651926/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1644920/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1623467/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1611079/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1589705/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:595128/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1550779/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1516716/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:647279/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:667404/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1454084/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:335500/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:464271/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:435438/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:42511/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:40624/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:3864/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:732276/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:782812/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:345269/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:68178/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:323763/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:310420/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:291364/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:517633/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:27145/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:266048/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:530708/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1419350/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1022538/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1028631/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1045908/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1053515/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1067055/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1081458/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:969921/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1109231/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1130437/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1132290/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1145833/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1159180/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1181463/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1192753/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:968178/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:934877/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1415738/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:879541/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:881652/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:914713/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1370093/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:136019/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1331180/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1310962/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:927277/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1301941/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1297634/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:1261746/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:698455/1‑62 (MQ=255)ctggaactggaaCATATGGGCCTGCCGTTCTCGCGTATTGATGATGGTCGTATCTATCAACg > 1:7116/1‑62 (MQ=255)ctggaactggaaCATATGGGCCGGCCGTTCTCGCGTCTTGATGATGGTCGTATCTATCAACg > 1:979857/1‑62 (MQ=255) gcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTGGAAAAACTTcggcg < 1:1566711/62‑1 (MQ=255) ggctcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:508376/58‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:761799/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:78881/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:797602/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:724459/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:980409/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:715040/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:840547/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:851460/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:856914/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:948792/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1003660/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1409663/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1013026/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1070057/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1101502/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1214358/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1227867/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1244509/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:130369/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1396109/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1404408/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:602173/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1439638/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1467950/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:1504553/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:250898/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:285905/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:366431/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:370812/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:486916/60‑1 (MQ=255) gTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg < 1:547923/60‑1 (MQ=255) | CTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCG > W3110S.gb/756617‑756710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |