| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 872,063 | 0 | T | C | 73.3% | 333.8 / 120.6 | 240 | R225R (CGT→CGC) | yliC | predicted peptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/64); new base C (0/176); total (0/240) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 240 total aligned reads displayed.
TGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGG > W3110S.gb/872026‑872107 | tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:532390/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:718736/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:705667/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1341696/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:653401/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:629777/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1399884/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1415846/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:625183/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1389529/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1522997/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:515197/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1610517/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:179841/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:43971/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:233076/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:315433/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:280889/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:93765/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1103086/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:894050/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1079945/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1061420/62‑1 (MQ=255)tGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1242826/62‑1 (MQ=255)tGAGAGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:1469417/62‑1 (MQ=255) gAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:549016/61‑1 (MQ=255) gAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:450478/61‑1 (MQ=255) gAGCGAAACCTGGGGTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGAcc < 1:377399/61‑1 (MQ=255) cTCAAACACGGTCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1474903/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:48571/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:966446/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:928850/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:499731/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:372543/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:955201/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:477868/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:976729/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:425343/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:406371/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:383834/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:833561/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:754747/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:731536/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:768385/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:777536/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:703237/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:791650/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:673314/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:798895/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:543084/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:20989/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:858239/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:871917/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:884651/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:62250/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:593674/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:586302/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1182955/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:272061/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1351557/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1327772/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1298748/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1296564/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1287458/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1232206/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1226243/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1214347/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:119153/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1356929/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:114607/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1139074/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1129504/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1096010/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1084606/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1073940/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1046027/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1034475/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1024651/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1511338/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1004260/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:204361/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:186141/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:183529/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:170498/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1619542/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1369717/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1574485/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:149501/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:235795/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:254873/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:148332/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1457423/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:14508/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1438424/62‑1 (MQ=255) cTCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1432897/62‑1 (MQ=255) tCAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:158527/61‑1 (MQ=255) cAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:763769/60‑1 (MQ=255) cAAACACGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:352580/60‑1 (MQ=255) acGGGCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACACTTTgg < 1:227114/55‑1 (MQ=255) ggCTACGCAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTgg < 1:1254244/52‑1 (MQ=255) | TGAGCGAAACCTGGGTTGTCCTCAAACACGGGCTACGTAACGCGATGATCCCGGTAGTGACCATGATGGGCTTACAGTTTGG > W3110S.gb/872026‑872107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |