Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,768,2460GA73.9% 287.9 / 113.5 222S718S (AGC→AGT) ydiJpredicted FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (58/0);  new base A (164/0);  total (222/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 222 total aligned reads displayed.

AAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCGG  >  W3110S.gb/1768205‑1768281
                                         |                                   
aagTTAAAGGTTTTCGGCGCGCGTTCCATCAGCGGTGCGTAACTCTCAAc                             >  1:354385/1‑50 (MQ=37)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACtctcaa                              >  1:207735/1‑47 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACtctcaa                              >  1:1049340/1‑47 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACtctcaa                              >  1:1385879/1‑47 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:335802/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:491297/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:465445/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:447065/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:435332/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:42922/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:427687/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:420175/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:394088/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:361782/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:517553/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:293288/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:281692/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:275701/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:260508/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1003155/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:217902/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:213727/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:174685/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:730338/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:959419/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:94213/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:923909/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:901095/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:895194/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:89084/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:865577/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:852177/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:820427/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:758591/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:512294/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:716218/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:697708/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:672607/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:664386/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:648173/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:609417/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:594994/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:549330/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:544945/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:242778/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1230250/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1380903/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1364063/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1357796/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1342006/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:129312/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:166904/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1284408/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1256026/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1248247/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1287760/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1188976/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1179778/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1169585/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1160983/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1131282/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1091525/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1035185/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:10059/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1394771/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1553627/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1538723/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1436160/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1389945/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:158885/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1635545/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1490630/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1631027/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1505018/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1527738/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1595427/1‑50 (MQ=38)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGCCCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:60359/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGCCGAGGTGGTCGCGCAGCgg  >  1:331190/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGCCGAGGTGGTCGCGCAGCgg  >  1:106434/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGctg  >  1:73635/1‑60 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1148181/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1234238/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1086596/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:818700/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:92106/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:802519/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:165619/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1579709/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1212107/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:658070/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:653743/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:621999/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1262910/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:570415/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:373962/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1325157/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1479971/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:477938/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1428284/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCg   >  1:204971/1‑61 (MQ=255)
                                         |                                   
AAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCGG  >  W3110S.gb/1768205‑1768281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑