Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,768,2520GA72.8% 270.6 / 114.1 213V716V (GTC→GTT) ydiJpredicted FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (58/0);  new base A (155/0);  total (213/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

AAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCGG  >  W3110S.gb/1768205‑1768281
                                               |                             
aagTTAAAGGTTTTCGGCGCGCGTTCCATCAGCGGTGCGTAACTCTCAAc                             >  1:354385/1‑50 (MQ=37)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:293288/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:512294/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:501602/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:473477/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:449097/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:446926/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:43286/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:42922/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:361782/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:335802/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:523283/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:281692/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:275701/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1003155/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:242778/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:214042/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:20586/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:174685/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:166904/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1635545/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:742809/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:959419/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:94213/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:923909/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:899808/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:894669/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:865577/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:852177/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:820427/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:758591/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:260508/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:730338/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:703732/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:669202/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:664386/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:648173/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:634261/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:609417/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:594994/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:546160/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1188976/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1381260/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:136683/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1342006/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1334720/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1311791/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1631027/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1286136/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1256026/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1230250/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1289310/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1179778/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1169585/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1162800/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1131282/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1091525/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1066787/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1062141/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1035185/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:10059/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1616799/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1595085/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1553627/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1483076/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1435707/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1392950/1‑50 (MQ=38)
aagTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGTGCGTAACTCTCAAc                             >  1:1415510/1‑50 (MQ=38)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGCCCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:60359/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGcg                      >  1:138961/1‑42 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGCCGAGGTGGTCGCGCAGCgg  >  1:331190/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:421690/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:484317/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:165619/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:412583/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:92106/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:905302/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1579709/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1086596/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1543197/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1158789/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:818700/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:802519/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:237508/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1530672/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1521428/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1499944/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:696156/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1212107/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1363001/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:658070/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:653743/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1234238/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1262910/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:373962/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1283663/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:570415/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:566919/1‑62 (MQ=255)
               ggCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCg   >  1:380691/1‑61 (MQ=255)
               ggCGCGCGTGCCATCAGCGGAGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCgg  >  1:1468115/1‑62 (MQ=255)
                                               |                             
AAGTTAAAGGTTTTCGGCGCGCGTGCCATCAGCGGCGCGTAGCTCTCGACCGTAGCGACGAGGTGGTCGCGCAGCGG  >  W3110S.gb/1768205‑1768281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑