Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,407,4600GA72.9% 308.2 / 127.0 240F356F (TTC→TTT) nuoCNADH:ubiquinone oxidoreductase, chain C,D
Reads supporting (aligned to +/- strand):  ref base G (18/47);  new base A (175/0);  total (193/47)
Fisher's exact test for biased strand distribution p-value = 2.17e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 241 total aligned reads displayed.

CGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAA  >  W3110S.gb/2407437‑2407510
                       |                                                  
cGGAACCACGCCGGGTGCATATGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1273948/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGCTTGCTTCCACCAGATCGTAAATTTTCTg              >  1:155804/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGAt                                         >  1:402693/1‑35 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGt                        >  1:1643617/1‑52 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTa                       >  1:224368/1‑53 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTc                >  1:744785/1‑60 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:1294421/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:1306712/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:686659/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:978705/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:653751/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:1624228/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:1523862/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCt               >  1:511786/1‑61 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:750254/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:744034/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:790660/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:240487/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:24843/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:26135/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:279229/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:824582/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:844526/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:343424/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:328469/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1537551/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:719584/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:704551/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:380970/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:663780/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:43174/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:462974/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:467226/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:650953/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:494263/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:50062/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:50625/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:536321/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:592276/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:610787/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1134776/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1229624/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:122275/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:12146/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1195705/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:981666/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1165003/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1152511/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1147949/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:955478/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1108133/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1102423/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:986346/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1071111/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1060768/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1039271/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:100274/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1402948/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1555088/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1517588/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:850331/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1498647/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1460609/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:865439/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1563766/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:884337/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1364500/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:95209/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1327361/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:131957/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1267979/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:1260861/1‑62 (MQ=255)
cGGAACCACGCCGGGTGCATACGAAAACCAGTGAATGCTTCCACCAGATCGTAAATTTTCTg              >  1:1284187/1‑62 (MQ=255)
cGGAACCACGCCGGGTGAATACGAAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTg              >  1:543323/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:993100/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:487367/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:1250840/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:1372996/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:1594151/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:1608136/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:212136/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:623079/1‑62 (MQ=255)
      cACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATc        >  1:351163/1‑62 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:853207/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1023026/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:109404/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1175242/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1191184/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1343679/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:920967/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:44127/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1412325/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1503788/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1568561/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:412488/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:390753/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:1627406/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:188159/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:375385/53‑1 (MQ=255)
                     cGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTaaa  <  1:722844/53‑1 (MQ=255)
                       |                                                  
CGGAACCACGCCGGGTGCATACGGAAACCAGTGATTGCTTCCACCAGATCGTAAATTTTCTGACGATCGGTAAA  >  W3110S.gb/2407437‑2407510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑