| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,623,238 | 0 | A | G | 68.3% | 264.4 / 149.1 | 240 | L513L (CTA→CTG) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base A (76/0); new base G (164/0); total (240/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 240 total aligned reads displayed.
CGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCA > W3110S.gb/2623216‑2623276 | cgccgcAAAACTCCCGCCGCCTGTTGTATGAAAtggtg > 1:1638303/1‑38 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGCCCGCGAAATCGc > 1:430781/1‑52 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATctc > 1:448886/1‑50 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1367703/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:252435/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:214614/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:324320/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:332403/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:404587/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1563471/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1553298/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:516102/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1359989/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:658977/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1284365/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:831790/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1009255/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1028756/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1072084/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:989600/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:878639/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:727960/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1161522/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:1178820/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCTCCAACGCgca > 1:793013/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:881240/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:683826/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:285080/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:308695/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:763560/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:681578/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:327326/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:362670/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:372243/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:380180/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:95082/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:61130/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:62957/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:857142/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:425226/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:622914/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:818573/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:993564/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:523917/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:54020/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:567185/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:59894/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1356458/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1018988/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:108033/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1134671/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1152059/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1189692/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1218675/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1253822/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1323931/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1326538/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1374031/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1461146/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:169064/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1639685/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:200472/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCGCCAACGCgca > 1:1537977/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACCGCGAAATCCCCAACGCgca > 1:1380238/1‑61 (MQ=255)cgccgcAAAACTCCCGCCGCCTGTTGTATGAAATGGTGGACAGAGAAATCTCCAACgcgc > 1:603511/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAATTGGTGGACCGCGAGATCGCCAACGCgca > 1:1104418/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGc > 1:1211075/1‑51 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCa > 1:1119892/1‑53 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACgcgc > 1:480996/1‑59 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:210612/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:297131/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:742237/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:750490/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:220110/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:175977/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:189904/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:978632/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:96936/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1271394/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:905892/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:921270/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1090116/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1604758/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1509090/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:45482/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:459284/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:494526/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1466898/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1427318/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:140494/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:585406/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:733702/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:243169/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1341791/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:652317/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:387702/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:668692/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:1525425/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgca > 1:700857/1‑60 (MQ=255) gccgcAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACAGCGAGATCGCCAACGCgca > 1:774551/1‑60 (MQ=255) | CGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCA > W3110S.gb/2623216‑2623276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |