Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,985,1140CT71.5% 207.4 / 96.8 172A204A (GCC→GCT) yqeGpredicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/49);  new base T (123/0);  total (123/49)
Fisher's exact test for biased strand distribution p-value = 3.50e-44
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 172 total aligned reads displayed.

GATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATA  >  W3110S.gb/2985081‑2985150
                                 |                                    
tctatGGATATAGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:222370/57‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1313197/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:20111/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:505714/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:677753/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:782513/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:672727/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:888436/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1555186/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:659283/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:544374/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:231229/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:559858/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:773323/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1348257/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:725600/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:31685/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1220759/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:11981/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:359892/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:44125/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1142649/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1038165/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:1101483/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:96514/53‑1 (MQ=255)
      gATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:974512/53‑1 (MQ=255)
       atatCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTa             <  1:323777/52‑1 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCAt                             >  1:278081/1‑35 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCattat                  >  1:858323/1‑46 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCTTTATTTCTACGTTTGCCATa  >  1:1288489/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTAc            >  1:1521835/1‑52 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTAc            >  1:1109078/1‑52 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1503692/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1587993/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1358559/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1279701/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1261183/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCAt   >  1:1013799/1‑61 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:68378/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:689925/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:628761/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:659266/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:641122/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:697523/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:629539/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:708890/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:990930/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:72432/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:734316/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:749254/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:755843/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:796502/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:819738/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:833735/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:854126/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:876852/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:900356/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:900362/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:915939/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:920553/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:934354/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:944604/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:95892/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1438873/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:610856/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:202514/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:179183/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:163700/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1587848/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1578193/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1567165/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:14719/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:211202/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1389043/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1374684/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1303124/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1237131/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1175356/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:10793/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:1014622/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:485277/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:602977/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:571225/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:565614/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:547137/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:536603/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:520131/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:513915/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:241456/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:480093/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:433390/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:411718/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:402622/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:400632/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:330831/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:265874/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:252338/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTATGCCATa  >  1:1068963/1‑62 (MQ=255)
        tatCGATTCCCGTGATGGTTTTCGCTTTTAGCCATACACCCATTATTTCTACGTTTGCCATa  >  1:920075/1‑62 (MQ=255)
        taacGATTCCCGTGATGGTTTTCGCTTTTAGCCATACGCCCATTATTTCTACGTTTGCCATa  >  1:15957/4‑62 (MQ=255)
                                 |                                    
GATATGGATATCGATTCCCGTGATGGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATA  >  W3110S.gb/2985081‑2985150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑