| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,618,848 | 0 | G | A | 67.6% | 317.2 / 225.3 | 331 | R167R (CGC→CGT) | rmuC | predicted recombination limiting protein |
| Reads supporting (aligned to +/- strand): ref base G (107/0); new base A (223/0); total (331/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 331 total aligned reads displayed.
TGGAGATTGCGAATTTCGTGGGTCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCG > W3110S.gb/3618816‑3618899 | tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt > 1:182083/1‑61 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:248763/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:413865/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:404760/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:39308/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:387124/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:364884/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:360826/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:35633/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:35229/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:268540/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:449990/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:212365/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:197059/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:176362/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1643514/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1627547/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1611100/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1603699/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1593766/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:778398/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:969658/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:907089/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:901777/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:868757/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:844376/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:826152/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:807749/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:790271/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:786580/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1014745/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:75459/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:747340/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:715326/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:698368/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:677404/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:648543/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:599292/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:565072/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1322510/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1173767/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1221938/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1156500/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1243766/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1258585/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1153726/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1276983/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1100283/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1313944/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:107889/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1318944/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1063646/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1329518/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1398421/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1425933/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1469019/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1496044/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1432818/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1421078/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1000719/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:150408/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:152866/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:1575844/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACAAAAGCTGTc > 1:422976/1‑62 (MQ=255)tGGAGGTTGCGGATTTCGTCAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt > 1:154692/1‑61 (MQ=255)tGGAGGTTGCGGATTTCGAGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc > 1:241592/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCt > 1:1204202/1‑37 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCt > 1:545939/1‑37 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAAcc > 1:630126/1‑61 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:757366/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1121007/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:767790/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1585585/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1132069/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1018799/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:795482/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:979062/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:967037/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1047721/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:887955/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:10279/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:920674/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:399987/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1447661/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:274807/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:301746/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:318993/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:332654/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1379360/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1369481/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1351654/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:409137/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1288404/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:466167/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:505479/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:530184/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:1266085/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCg > 1:611028/1‑62 (MQ=255) tCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAAACTGACGGCGGAAAcc > 1:657379/1‑61 (MQ=255) tCAGGGTATGGAGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAAcc > 1:688068/1‑61 (MQ=255) | TGGAGATTGCGAATTTCGTGGGTCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTCCTGAACCTGACGGCGGAAACCG > W3110S.gb/3618816‑3618899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |