Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,946,9080CT73.3% 356.9 / 140.6 274T576T (ACC→ACT) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (73/0);  new base T (0/200);  total (73/201)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

TGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCT  >  W3110S.gb/3946896‑3946957
            |                                                 
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATc   >  1:630571/1‑61 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:283973/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:522665/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1464353/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1519908/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1438647/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1433201/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:226552/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:530411/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1400130/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:759146/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:259498/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1556708/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1338643/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1286569/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:125555/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:608980/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:95329/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1037959/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1051799/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1063985/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1074217/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:932234/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:924842/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:917014/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1184633/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:413408/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCGGGAGTGAAATCt  >  1:1500712/1‑62 (MQ=255)
tGCCAGCCTGACCAACTACAACATCCAGGGCAAataa                           >  1:1602676/1‑36 (MQ=37)
   cAGCCTGACTAACTCGAAGATGCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1458645/52‑1 (MQ=255)
    aGCCTGATTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1020576/51‑1 (MQ=255)
    aGCCTGACTAACTCGAAGATCTAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:506911/51‑1 (MQ=255)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:566085/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:540743/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:454412/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:503856/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:478450/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:394395/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:429566/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:42441/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:419733/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:408889/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:97238/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:773113/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1001525/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:887960/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:879569/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:857529/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:843618/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:836835/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:802928/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:785536/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:574384/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:742916/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:717621/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:687964/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:673563/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:661892/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:654111/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:597406/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:59203/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1237630/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:369333/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1470925/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1446633/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1428892/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1412752/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1369109/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1353764/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1311694/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1264692/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1542937/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1222954/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:120226/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1190915/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1166696/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1148795/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:110598/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1026180/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1006428/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:197542/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:358821/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:355391/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:347422/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:318284/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:273854/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:266377/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:247249/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:216781/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:207884/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1585167/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:176244/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:167053/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1639852/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1631964/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1624222/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1607569/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:159676/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATAT‑‑CCACTCTTTCTGGAGt         <  1:1507813/49‑1 (MQ=25)
        tGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1346724/47‑1 (MQ=38)
            |                                                 
TGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCT  >  W3110S.gb/3946896‑3946957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑