Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,946,9140CG73.5% 435.6 / 179.8 276S578S (TCC→TCG) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (73/0);  new base G (0/202);  total (73/203)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 276 total aligned reads displayed.

TGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCT  >  W3110S.gb/3946896‑3946957
                  |                                           
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATc   >  1:630571/1‑61 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATc   >  1:1279856/1‑61 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:259498/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:283973/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:280837/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1338643/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:781680/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1361762/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1400130/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:759146/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1556708/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1433201/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1438647/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:608980/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:226552/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1464353/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1519908/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:125555/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:95329/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1051799/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1063985/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1074217/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:932234/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:413408/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:917014/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1184633/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:356920/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:856164/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCGGGAGTGAAATCt  >  1:1500712/1‑62 (MQ=255)
tGCCAGCCTGACCAACTCCAAGATCAAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCt  >  1:1040921/1‑62 (MQ=255)
tGCCAGCCTGACCAACTACAACATCCAGGGCAAataa                           >  1:1602676/1‑36 (MQ=37)
    aGCCTGATTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1020576/51‑1 (MQ=255)
    aGCCTGACTAACTCGAAGATCTAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:506911/51‑1 (MQ=255)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:566085/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:432960/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:534122/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:529778/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:516704/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:490872/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:47209/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:687964/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:42441/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:419733/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:409737/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:742916/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1001525/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:922907/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:887960/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:879569/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:843618/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:836835/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:802928/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:773113/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:574384/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:704155/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:97238/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:673563/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:661892/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:645737/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:597406/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:585551/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1231657/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1507320/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1470925/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:145772/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1446633/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1428225/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1412752/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1351699/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1308744/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1257823/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1542937/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1216463/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:120226/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1197145/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1166696/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1148795/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:111539/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1026180/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1006428/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:197542/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:369333/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:358821/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:347422/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:318284/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:266377/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:247249/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:216781/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:207884/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:394395/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:167053/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1639852/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1631964/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1620336/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1607569/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:159676/51‑1 (MQ=39)
    aGCCTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:157999/51‑1 (MQ=39)
    aGCCAGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:1343384/51‑1 (MQ=37)
      ccTGACTAACTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:176057/49‑1 (MQ=38)
               cTCGAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGt         <  1:451011/40‑1 (MQ=255)
                  |                                           
TGCCAGCCTGACCAACTCCAAGATCCAGGGCAAATATCGCCACTCTTTCTGGAGTGAAATCT  >  W3110S.gb/3946896‑3946957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑