| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,996,224 | 0 | G | A | 73.0% | 274.1 / 117.6 | 226 | Q330Q (CAG→CAA) | prlC | oligopeptidase A |
| Reads supporting (aligned to +/- strand): ref base G (11/50); new base A (165/0); total (176/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.84e-40 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 226 total aligned reads displayed.
CGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGC > W3110S.gb/3996183‑3996275 | cGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac > 1:278279/1‑60 (MQ=255)cGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac > 1:841556/1‑60 (MQ=255)cGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac > 1:83137/1‑60 (MQ=255)cGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTtac > 1:314025/1‑60 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCg > 1:1043268/3‑60 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:324664/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:519729/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:505061/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:493213/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:469876/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:456595/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:408001/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:406470/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:394325/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:381965/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:35265/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:333495/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:576911/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:30033/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:271866/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1011149/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:249485/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:221193/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:210829/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:730559/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:982544/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:966467/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:957411/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:9503/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:918552/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:8947/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:860503/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:782650/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:758604/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:738100/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:545551/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:667057/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:656192/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:630820/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:624961/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:610437/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:599281/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:591291/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:26701/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:553210/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:120986/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1424127/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1383120/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1370522/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:206566/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1342186/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1326336/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1306170/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1290178/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1272092/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1257264/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:123473/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1353851/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1201803/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1168473/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1159435/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1139717/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1127797/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1108410/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1078373/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1073635/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1035974/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1025667/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:201466/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1629800/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1651326/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1612597/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1584999/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:171319/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1558436/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1546110/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1535020/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1521102/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1507836/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1478455/3‑61 (MQ=255) tcgCCAAAGCCGAATTTGGCGTCGATGAGTTACAACCGTGGGATATCGCTTACTACAGCGa > 1:1464293/3‑61 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:940267/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:227563/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:93008/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1063862/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:905239/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:19837/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1640847/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1604579/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:273663/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:485172/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1232464/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:378540/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1335346/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1377152/61‑1 (MQ=255) tGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:1436641/61‑1 (MQ=255) gtgTTGCAGCCGTGGGATATCGCTTACTAGAGCGAAATACATAAACAGCACCTCTACAGc < 1:439854/58‑1 (MQ=255) gAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:805794/60‑1 (MQ=255) gAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:795929/60‑1 (MQ=255) gAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGc < 1:714709/60‑1 (MQ=255) | CGTGCCTTTGCCAAAGCCGAATTTGGCGTCGATGAGTTGCAGCCGTGGGATATCGCTTACTACAGCGAAAAACAAAAACAGCACCTCTACAGC > W3110S.gb/3996183‑3996275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |