| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 86,504 | C→A | A292E (GCA→GAA) | ilvI → | acetolactate synthase III, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 86,504 | 0 | C | A | 100.0% | 10.9 / NA | 6 | A292E (GCA→GAA) | ilvI | acetolactate synthase III, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/6); total (0/6) | |||||||||||
ATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCC > W3110S.gb/86455‑86515 | aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:1006120/61‑1 (MQ=255)aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:1053530/61‑1 (MQ=255)aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:1347044/61‑1 (MQ=255)aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:818721/61‑1 (MQ=255)aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:924027/61‑1 (MQ=255)aTTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGAAAAGTACTGcc < 1:973352/61‑1 (MQ=255) | ATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCC > W3110S.gb/86455‑86515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |