Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 1,060,340 | C→T | N221N (AAC→AAT) | torA → | trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,060,340 | 0 | C | T | 89.8% | 356.6 / 20.2 | 157 | N221N (AAC→AAT) | torA | trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit |
Reads supporting (aligned to +/- strand): ref base C (0/16); new base T (0/141); total (0/157) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 |
Only 100 of 157 total aligned reads displayed.
GAAGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGAAAAA > W3110S.gb/1060296‑1060381 | aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:528981/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:153590/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:1395445/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:314116/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:210115/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:882638/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:1337307/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:1646525/61‑1 (MQ=255) aGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCtg < 1:832241/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCCAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1591054/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGTCTCCGATTTGCTGaaaaa < 1:274193/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaata < 1:774859/61‑3 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:322323/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:547981/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:275305/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:512899/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:512710/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:505129/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:311053/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:475057/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:468535/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:364685/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:448199/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:427965/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:411259/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:383889/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:349167/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:38047/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:364099/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:760597/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:960139/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:944610/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:930869/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:918588/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:901811/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:875234/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:843499/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:83237/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:822352/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:81173/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:782834/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:514071/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:714920/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:685620/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:683425/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:634217/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:622526/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:592687/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:591860/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:572671/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:270983/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:53241/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1233436/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:101114/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1387760/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1387297/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:138407/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1375845/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1357274/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1348095/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1346757/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1320510/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1316273/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1265712/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1430331/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1203469/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:119910/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1194128/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1185442/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1173683/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1099500/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1095052/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1078976/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1042644/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:101331/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1575883/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:240114/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:239486/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:196190/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:185970/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:178765/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:170831/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:163908/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1628986/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:162691/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1621421/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1613938/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1431152/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1550551/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1543920/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:153642/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1495602/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:147780/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1455774/61‑1 (MQ=255) ggCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1442482/61‑1 (MQ=255) ggCCGATGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:1569475/61‑1 (MQ=255) gCCGCTGGTATTGCATAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:707599/60‑1 (MQ=255) gCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:653470/60‑1 (MQ=255) gCCGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:54880/60‑1 (MQ=255) ccGCTGGTATTGCAGAATAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGaaaaa < 1:327961/59‑1 (MQ=255) | GAAGTGTATGAACAGCAAACCTCCTGGCCGCTGGTATTGCAGAACAGCAAAACCATTGTGCTGTGGGGCTCCGATTTGCTGAAAAA > W3110S.gb/1060296‑1060381 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |