| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 108,070 | C→T | L122L (CTC→CTT) | secM → | regulator of secA translation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 108,070 | 0 | C | T | 99.6% | 721.8 / ‑6.3 | 249 | L122L (CTC→CTT) | secM | regulator of secA translation |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (248/0); total (249/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 249 total aligned reads displayed.
CGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTC > W3110S.gb/108042‑108102 | cgcAACATCTTGCATTACTGGATACGCTTAGCGCGcttctg > 1:678485/1‑41 (MQ=38)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGa > 1:646004/1‑42 (MQ=38)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACGCAGGAAGGCACGCCGTc > 1:391696/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCGGGAAGGCACGCCGTc > 1:861937/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCa > 1:1320207/1‑54 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGt > 1:1263950/1‑60 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:460397/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:563161/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:542247/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:529063/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:515953/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:510117/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:506581/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:492221/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:488718/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:476801/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:623333/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:441801/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:432216/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:427559/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:39995/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:375484/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:354767/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:338108/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:308326/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:297798/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:292534/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:281574/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:800741/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:996182/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:981711/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:95576/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:950159/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:932848/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:91795/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:900567/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:884934/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:8643/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:842403/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:81724/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:579576/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:786241/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:747628/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:732701/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:721795/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:704456/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:698071/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:691091/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:269/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:608910/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:597636/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1244546/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1007406/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1398338/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1391882/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1383282/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1371162/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1362993/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1303116/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1292933/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:128621/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1256778/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1247998/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1416618/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1228381/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1203401/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1174494/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1169001/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1134938/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1131320/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1102345/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1063653/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1039816/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1028768/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1019091/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1433284/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:260221/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:256176/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:242885/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:205976/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:204291/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:198284/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:184554/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:168397/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1645643/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1639712/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1626840/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1592089/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1546938/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1526395/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1512721/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1503190/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1481268/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1481063/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:145036/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:1439210/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:14317/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTATGACCCAGGAAGGCACGCCGTc > 1:1095855/1‑61 (MQ=255)cgcAACATCTTGCATTACTGGATACGCTTAGCGCGATTCTGACACAGGAAGGCACGCCGTc > 1:161201/1‑61 (MQ=255)cgcAACATATTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc > 1:762519/1‑61 (MQ=255) | CGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTC > W3110S.gb/108042‑108102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |