| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,220,283 | G→A | G460D (GGT→GAT) | ycgG → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,220,283 | 0 | G | A | 92.8% | 234.8 / 4.6 | 97 | G460D (GGT→GAT) | ycgG | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (7/0); new base A (90/0); total (97/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTCAGCAG > W3110S.gb/1220245‑1220341 | tatGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATCTCGAAACGAAAGaaca > 1:1011409/3‑57 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGatg > 1:1248386/1‑41 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:99861/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:328327/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:1382828/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:1301122/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:607943/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAAttt > 1:1289821/1‑61 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:311629/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:542911/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:524029/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:5049/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:494222/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:483017/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:473829/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:989047/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:333410/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:329074/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:166778/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:551639/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:289929/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:287924/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:287566/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:258711/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:258296/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:217460/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:217071/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:176730/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:176721/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:69134/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:918579/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:858377/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:820952/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:818257/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:784126/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:754293/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:745731/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:953070/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:736109/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:696935/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:947329/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:686537/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:969795/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:67382/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:671690/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:624781/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:969906/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:974849/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:575306/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:565736/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1164682/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1310255/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1303909/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1004399/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1297245/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1291448/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1270886/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1261783/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1245469/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1236179/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1177668/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1300001/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1157067/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1135799/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1133963/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1110023/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1076656/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1038658/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1026800/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1026039/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:100648/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1384777/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1617638/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1614327/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1607428/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1607102/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:154858/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1488132/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1470956/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1470329/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1426168/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1414407/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1398810/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1316729/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1355366/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1353248/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1334862/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1328212/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1327294/1‑62 (MQ=255)tCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:1323805/1‑62 (MQ=255)tCTGGCGCGTAAACATTCGATCAGTATCGTCGCTGAAGATGTCGAAACGAAAGAACAATTTg > 1:988673/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:445641/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:736311/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:127152/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:1272545/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:1297084/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:1022413/1‑62 (MQ=255) aaGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTcagcag > 1:574896/1‑62 (MQ=255) | TCTGGCGCGTAAACTTTCGATCAGTATCGTCGCTGAAGGTGTCGAAACGAAAGAACAACTTGACTATCTGAACCAAAATTATATCACATTTCAGCAG > W3110S.gb/1220245‑1220341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |