Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 108,079 G→T L125L (CTG→CTT)  secM → regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb108,0790GT100.0% 707.7 / NA 244L125L (CTG→CTT) secMregulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (244/0);  total (244/0)

Only 100 of 246 total aligned reads displayed.

CGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTC  >  W3110S.gb/108042‑108102
                                     |                       
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCGGGAAGGCACGCCGTc  >  1:861937/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGc      >  1:285783/1‑57 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGt   >  1:505372/1‑60 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGt   >  1:1263950/1‑60 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:436933/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:565645/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:558185/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:537359/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:522568/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:514610/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:509648/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:490777/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:481061/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:474925/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:453751/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:603110/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:432216/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:424562/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:39995/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:391310/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:375484/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:347781/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:338108/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:303858/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:297798/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:281574/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:800741/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:996182/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:981711/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:95576/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:950159/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:932848/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:91795/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:900567/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:884934/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:8643/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:821010/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:807380/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:58363/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:778417/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:740000/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:732701/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:717580/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:704456/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:693355/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:685633/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:647814/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:639190/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:609764/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:269/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1247998/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1416618/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1007406/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1392375/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1383282/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1371949/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1362993/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1337904/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1303116/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1296269/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1285477/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1254225/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1398338/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1228381/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1203401/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1174494/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1169001/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1134938/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1131320/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1102345/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1063653/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1039816/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1028768/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1019091/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1433284/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:259823/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:256176/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:23206/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:205976/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:203534/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:198284/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:178255/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:168397/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1643614/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1639712/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:162190/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1592089/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1546938/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1526395/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1512721/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1503190/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1481268/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1481063/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:145036/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:1439210/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:14317/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGCTTATGACCCAGGAAGGCACGCCGTc  >  1:1095855/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCTTAGCGCGATTCTGACACAGGAAGGCACGCCGTc  >  1:161201/1‑61 (MQ=255)
cgcAACATCTTGCATTACTGGATACGCATAGCGCGCTTCTGACCCAGGAATGCACGCCgac  >  1:1244542/1‑59 (MQ=255)
cgcAACATATTGCATTACTGGATACGCTTAGCGCGCTTCTGACCCAGGAAGGCACGCCGTc  >  1:762519/1‑61 (MQ=255)
                                     |                       
CGCAACATCTTGCATTACTGGATACGCTCAGCGCGCTGCTGACCCAGGAAGGCACGCCGTC  >  W3110S.gb/108042‑108102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑