Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,503,086	T→A	L267Q (CTG→CAG)	tehA →	potassium‑tellurite ethidium and proflavin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,503,086	0	T	A	100.0%	54.1 / NA	21	L267Q (CTG→CAG)	tehA	potassium‑tellurite ethidium and proflavin transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/0);  total (21/0)

TCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACTGGCAACCACCGGTTTGCATCTGGGGAG  >  W3110S.gb/1503052‑1503113                                   |                            tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGc                          >  1:79003/1‑38 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAATCACCGGTTTGCATCTGGGGAg  >  1:47704/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCg                  >  1:668647/1‑46 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:515919/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:939410/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:806278/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:766748/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:704623/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:687741/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:670592/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:592587/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:56224/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:1008945/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:461679/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:350965/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:228606/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:155210/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:1397075/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:1252525/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:1059242/1‑62 (MQ=255) tCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACAGGCAACCACCGGTTTGCATCTGGGGAg  >  1:1028534/1‑62 (MQ=255)                                   |                            TCATTCTGGAGTTTCTCGTTCGGCGTATCTGCACTGGCAACCACCGGTTTGCATCTGGGGAG  >  W3110S.gb/1503052‑1503113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑