| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,515,238 | T→G | G236G (GGT→GGG) | ydcT → | predicted spermidine/putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,515,238 | 0 | T | G | 99.5% | 619.8 / ‑4.4 | 211 | coding (708/1014 nt) | ydcT | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (210/0); minor base . (1/0); total (211/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 211 total aligned reads displayed.
CCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGAT > W3110S.gb/1515205‑1515264 | cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGa > 1:929494/1‑41 (MQ=25)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATTTTTTTGATGGACTGAt > 1:840565/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGtttt > 1:45282/1‑48 (MQ=37)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTg > 1:810934/1‑50 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATg > 1:785000/1‑53 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACt > 1:801022/1‑57 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTTAt > 1:144185/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGa > 1:1570211/1‑59 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGa > 1:531030/1‑59 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGa > 1:67723/1‑59 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGa > 1:1280015/1‑59 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGa > 1:456631/1‑59 (MQ=38)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:504012/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:483576/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:470689/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:529654/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:550395/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:556078/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:57470/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:643262/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:444104/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:438093/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:401257/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:378032/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:373997/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:360851/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:346136/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:335058/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:322118/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:307709/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:837780/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:99236/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:983821/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:969129/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:949769/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:946611/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:939430/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:932303/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:918191/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:893267/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:849539/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:583684/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:825618/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:820973/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:793957/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:761837/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:736414/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:712261/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:658291/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:287158/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:623623/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:613821/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1224074/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:297828/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:137635/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1368374/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1343885/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1328135/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:129249/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1267150/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1262931/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1258316/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1254807/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1249154/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1384633/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1174908/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1171176/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1160170/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1159433/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1143179/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1121858/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1117482/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1084955/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1068804/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1057665/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1037472/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1583584/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1016360/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:269702/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:23134/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:221409/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:198350/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:197674/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:178739/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1642683/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1628076/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1616479/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1394041/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1550029/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1518483/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1503638/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1493733/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1480324/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:14592/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1432908/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:142679/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:142079/1‑60 (MQ=255)cccGCGCACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1399793/1‑60 (MQ=255)cccGCGCACGCCGTTTGGTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:249711/1‑60 (MQ=255)ccagcgcACGCCGTTTGTTGCGGGATTCGTTGGGACATCGAATGTTTTTGATGGACTGAt > 1:1233498/4‑60 (MQ=255) | CCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGAT > W3110S.gb/1515205‑1515264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |