Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,578,992 A→C T114T (ACT→ACG)  yddB ← predicted porin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,578,9920AC100.0% 1017.2 / NA 346T114T (ACT→ACG) yddBpredicted porin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (346/0);  total (346/0)

Only 100 of 346 total aligned reads displayed.

GTTCAGGTTATTAGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCACC  >  W3110S.gb/1578980‑1579042
            |                                                  
gTTCAGGTTATTCGTTGCACTAATACCTTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:59106/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAGATAGGCATTCTGGTAGGGCGACGCAc   >  1:52944/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATa                          >  1:1120633/1‑39 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGc          >  1:1326655/1‑55 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCg         >  1:1008554/1‑56 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:748934/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:1217188/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:1573083/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:253258/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:414813/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:896678/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCa    >  1:950193/1‑61 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:555967/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:541511/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:562477/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:575539/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:535779/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:325063/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:599708/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:516660/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:494451/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:483878/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:456902/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:435034/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:430034/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:421209/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:401725/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:386977/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:347732/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:287498/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:778726/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:993075/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:971373/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:931120/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:907653/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:889066/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:879714/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:843294/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:83257/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:826756/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:81582/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:806223/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:603450/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:756815/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:736521/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:725419/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:693117/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:682735/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:673929/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:659668/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:65511/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:651474/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:623509/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1203033/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1414014/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1391237/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1384043/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1361058/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1342549/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1332692/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1301976/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1271774/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1256705/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1250493/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1232505/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:300949/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1193085/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1183301/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1170358/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1163628/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1143386/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1087819/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1082301/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1059660/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1050595/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1016755/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1461286/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1001557/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:279543/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:261153/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:205539/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:196814/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:179634/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:173640/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1644833/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1634520/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1612395/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1597600/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1555531/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1543500/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1534374/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1530486/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1515293/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1498256/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1480175/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1469674/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCAc   >  1:1450957/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCCACGCAc   >  1:225641/1‑62 (MQ=255)
gTTCAGGTTATTCGTTGCACTAATACCGACAATCAAATAGGCAt                     >  1:995909/1‑44 (MQ=255)
gTTCAGGTTATTCGTTGAACTAATACCGTCAATCAAATAGGCa                      >  1:310405/1‑43 (MQ=38)
            |                                                  
GTTCAGGTTATTAGTTGCACTAATACCGTCAATCAAATAGGCATTCTGGTAGGGCGACGCACC  >  W3110S.gb/1578980‑1579042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑