Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,615,140	G→A	D426D (GAC→GAT)	yneI ←	predicted aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,615,140	0	G	A	100.0%	227.9 / NA	80	D426D (GAC→GAT)	yneI	predicted aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/80);  total (0/80)

TTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCAC  >  W3110S.gb/1615114‑1615174                           |                                   ttCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1101031/61‑1 (MQ=255)  tCACTCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:552423/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCCTTGATAAACACCCCAc  <  1:1571115/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:380435/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:572690/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:552803/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:519413/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:497436/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:495914/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:453386/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:388671/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:573500/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:358122/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:33961/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:319372/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:312192/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:306211/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1035492/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:284865/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:280402/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:787614/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:997856/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:944334/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:941793/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:936144/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:927614/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:865791/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:819974/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:790768/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:290922/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:733953/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:733151/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:690667/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:667848/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:637206/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:620203/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:60700/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:597243/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1257472/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1503226/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1496477/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1460199/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1435834/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:142796/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1392261/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1338676/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1258267/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:144602/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1239056/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1234863/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:117696/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:115402/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1146465/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1084857/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1041845/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1534105/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:240926/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:226598/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:209098/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:193482/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1871/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:161920/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1602911/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1599554/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1594574/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1578866/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:155863/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1553636/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1514187/60‑1 (MQ=255)  tCACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGAAAAACACCCCAc  <  1:1081521/60‑1 (MQ=255)   cACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:795276/59‑1 (MQ=255)   cACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:277289/59‑1 (MQ=255)   cACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:840610/59‑1 (MQ=255)   cACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:929976/59‑1 (MQ=255)   cACGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:258788/59‑1 (MQ=255)    aCGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:857493/58‑1 (MQ=255)     cGCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:542413/57‑1 (MQ=255)      gCCACCAAAGGCGACTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:517114/56‑1 (MQ=255)                 cgaCTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:1103337/43‑1 (MQ=39)                    cTCGCGCATCGCTGGCACAATAACCATTGATAAACACCCCAc  <  1:861765/42‑1 (MQ=255)                           |                                   TTCACGCCACCAAAGGCCACTCGCGCGTCGCTGGCACAATAACCATTGATAAACACCCCAC  >  W3110S.gb/1615114‑1615174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑