Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,655,312	C→A	L106L (CTG→CTT)	rspB ←	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,655,312	0	C	A	100.0%	16.9 / NA	8	L106L (CTG→CTT)	rspB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/8);  total (0/8)

CGGCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCCAGTG  >  W3110S.gb/1655266‑1655316                                               |     gagCATATTCACTGAAACCACCGTCAGCTTGCACACCTAATACAGCAAgtg  <  1:869469/49‑1 (MQ=255) cagCATATTCTCTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:1309087/49‑1 (MQ=255) cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:1619055/49‑1 (MQ=255) cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:327359/49‑1 (MQ=255) cagCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:733408/49‑1 (MQ=255) cagCACATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:545809/49‑1 (MQ=255)     atatTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:1256162/47‑1 (MQ=255)            cTGAAACCACCGTCAGCGTGCACACCTAATACAGCAAgtg  <  1:1012846/40‑1 (MQ=255)                                               |     CGGCATATTCACTGAAACCACCGTCAGCGTGCACACCTAATACAGCCAGTG  >  W3110S.gb/1655266‑1655316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑