| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,699,047 | C→G | A236A (GCG→GCC) | hdhA ← | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,699,047 | 0 | C | G | 97.7% | 365.3 / ‑2.5 | 128 | A236A (GCG→GCC) | hdhA | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (3/0); new base G (125/0); total (128/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
GCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG > W3110S.gb/1698995‑1699072 | gttCCTGTCCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:478127/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGGATTTGTCCGCTTACCCAGCTGGctgcag > 1:544643/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTCCCCAGCTGGctgcag > 1:863863/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca > 1:601267/3‑59 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca > 1:1599571/3‑59 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca > 1:1617501/3‑59 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGgctgca > 1:1341493/3‑59 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:485587/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:59119/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:575131/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:566095/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:555901/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:549780/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:533416/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:527708/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:520995/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:284917/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:469877/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:466543/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:434311/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:420747/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:369564/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:339387/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:336049/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:331176/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:325200/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:298138/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:262543/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:76133/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:951141/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:913936/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:882132/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:859806/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:853540/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:850741/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:823715/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:79992/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:791573/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:773136/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:770971/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:596540/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:746604/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:739705/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:712718/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:706613/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:67342/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:667114/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:658644/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:657737/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:631346/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:629846/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:60619/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1267703/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1438728/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1432476/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1428980/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1422308/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1413759/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1405534/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1399770/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1385479/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1363769/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1281795/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1279266/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1461673/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1258023/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1183453/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1178154/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1174750/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1132964/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1131231/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1125285/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1103491/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1065157/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1049954/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1019245/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1622445/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:100243/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:245982/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:244402/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:23037/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:222634/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:221274/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:219379/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:204791/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:180141/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:178482/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:175751/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:264760/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1601096/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1593984/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1587786/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1586601/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1581577/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1580359/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:156438/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1519732/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1488665/3‑60 (MQ=255)gttCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag > 1:1482254/3‑60 (MQ=255) accGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc > 1:1577707/1‑61 (MQ=255) | GCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCG > W3110S.gb/1698995‑1699072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |