Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,726,328	T→C	A14A (GCA→GCG)	sodC ←	superoxide dismutase, Cu, Zn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,726,328	0	T	C	100.0%	269.6 / NA	93	A14A (GCA→GCG)	sodC	superoxide dismutase, Cu, Zn
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (93/0);  total (93/0)

TTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATA  >  W3110S.gb/1726290‑1726349                                       |                      ttCATCTCGAGTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAt   >  1:1272978/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGcaacaa                >  1:970842/1‑46 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGaagg  >  1:1478973/1‑58 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGaa    >  1:1177499/1‑58 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:489094/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:694897/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:682416/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:656278/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:645065/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:634020/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:633866/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:62516/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:606478/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:569319/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:537664/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:70804/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:484249/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:467935/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:41483/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:366035/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:337552/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:316286/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:3160/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:300666/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:263326/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:825246/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:978956/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:96690/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:959056/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:943088/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:941708/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:938553/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:919378/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:91083/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:884923/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:867039/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:699262/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:821723/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:820238/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:816789/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:81515/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:787214/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:786207/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:726018/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:722067/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:249106/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1248985/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1408157/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1407463/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:140716/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1403668/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1385060/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1369771/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1355038/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1330916/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1330276/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1259857/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1253557/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1412740/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1245881/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1228272/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1220984/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1169024/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1157055/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1103212/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:107946/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1071422/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1045562/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1024393/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:101881/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1417603/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:220122/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:177600/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:170644/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1640331/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1633567/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:160027/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1587260/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1569197/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1542740/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1522126/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1508984/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1483570/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1478692/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1467924/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1449005/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:144618/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1431971/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:1010323/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAt   >  1:260736/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAAACAGCCCCAGAAtg  >  1:61549/1‑59 (MQ=255) ttCATCTCGACTTTTTCACTGGCAGCTTGTGAGCCGGTCGcaacaa                >  1:449406/1‑46 (MQ=39) ttCATCACGACTTTTTCACTGGCAGCTTGTGCGCCGGTCGCAACAACCAGCGCCAGAAtg  >  1:421976/1‑59 (MQ=255)                                       |                      TTCATCTCGACTTTTTCACTGGCAGCTTGTGCGCCGGTTGCAACAACCAGCGCCAGAATA  >  W3110S.gb/1726290‑1726349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑