Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	136,240	A→G	H75H (CAT→CAC)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	136,240	0	A	G	100.0%	270.7 / NA	93	H75H (CAT→CAC)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (93/0);  total (93/0)

ACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAA  >  W3110S.gb/136223‑136284                  |                                             acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGTACATGGGTCATCATCTCATGATAGATaaa  >  1:1103682/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGtcatc                   >  1:1557662/1‑45 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGt                       >  1:365839/1‑41 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCa             >  1:295094/1‑51 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagataga  >  1:108209/1‑60 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATgatagata    >  1:958161/1‑60 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGTTAGATaaa  >  1:890656/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:437033/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:656326/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:647553/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:635891/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:627849/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:599936/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:577913/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:564551/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:552201/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:54463/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:536956/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:494429/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:685971/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:384333/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:383545/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:369456/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:330942/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:315471/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:278782/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:75993/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:995742/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:982343/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:980063/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:963621/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:95033/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:821078/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:821041/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:784450/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:780341/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:779311/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:658144/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:755251/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:753828/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:753276/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:742788/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:72018/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:713275/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:691878/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1018064/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:670291/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1167492/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1559600/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:275318/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1514502/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:150766/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1413230/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:140694/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1402006/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:125438/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1222262/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1189036/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1521545/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1140059/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1130571/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1122096/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1095654/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1078445/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:106225/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1039012/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1030305/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:100998/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:163531/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:191534/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:187610/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:185314/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:183666/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:205009/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:210376/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1650760/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:217954/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1630270/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1629231/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:222270/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:234769/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1597989/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1574797/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1570045/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaaa  >  1:1560723/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa   >  1:1559070/1‑61 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa   >  1:506860/1‑61 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa   >  1:53116/1‑61 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa   >  1:1352721/1‑61 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATaa   >  1:1483254/1‑61 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGTAGCGGAACATGGGTCATCATCTCATCATAGATaaa  >  1:1501206/1‑62 (MQ=255) acaTGTTTCGCGTGGCCGTGGGCCAGGAGCGGAACAt                           >  1:1045243/1‑37 (MQ=25) acaTGTTTCGCGTAGCCGTGGGCCAGTAGCGGAACATg                          >  1:266361/1‑38 (MQ=37)                  |                                             ACATGTTTCGCGTGACCATGGGCCAGTAGCGGAACATGGGTCATCATCTCATGATAGATAAA  >  W3110S.gb/136223‑136284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑