Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,894,779	A→G	A57A (GCT→GCC)	yoaA ←	conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,894,779	0	A	G	100.0%	246.8 / NA	85	A57A (GCT→GCC)	yoaA	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (85/0);  total (85/0)

ATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGC  >  W3110S.gb/1894750‑1894790                              |            aagACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1049454/3‑41 (MQ=38) aTGACTTTCTTTTTCGCCCGCAGCTCAGGGGCCAGGTAAGc  >  1:174986/1‑41 (MQ=37) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAg   >  1:1598785/1‑40 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAg   >  1:88593/1‑40 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAg   >  1:87456/1‑40 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAg   >  1:1621735/1‑40 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:658987/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:724616/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:657/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:619804/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:593740/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:574369/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:557828/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:507408/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:502121/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:492665/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:476814/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:472575/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:468746/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:463696/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:454104/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:450212/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:422449/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:390882/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:844752/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:996701/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:992324/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:969966/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:963915/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:962522/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:926691/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:908038/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:906792/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:899977/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:718932/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:830790/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:816858/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:816267/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:816067/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:78977/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:780405/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:773545/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:734704/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:37066/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1251541/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:136805/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1352055/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1347537/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1338790/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1321866/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1310174/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1309428/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1296397/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1266846/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1259639/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1399662/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1247159/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1236671/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1215595/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1198114/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1111688/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1104591/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1104409/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1082470/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1031538/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1418224/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:374134/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1019677/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:370318/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:362820/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:336775/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:322292/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:229770/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:208133/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1645484/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1602752/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:15784/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1530493/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1494662/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1472538/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1464523/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1459743/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:1430932/1‑41 (MQ=255) aTGACTTTCTTTTTCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:375261/1‑41 (MQ=255) aTGACTTTCTTTTCCGCCCGCAGCGCAGGGGCCAGGTAAGc  >  1:38748/1‑41 (MQ=38)                              |            ATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGC  >  W3110S.gb/1894750‑1894790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑