Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,973,179 G→T A409A (GCC→GCA)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,973,1790GT100.0% 318.5 / NA 119A409A (GCC→GCA) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (119/0);  total (119/0)

Only 100 of 119 total aligned reads displayed.

TTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGG  >  W3110S.gb/1973168‑1973222
           |                                           
tttGCCGCCTGTGCACTGCGACTGGCAAGCTTACGCACTTCACCCGCCACCACg   >  1:228153/1‑54 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCa               >  1:982356/1‑42 (MQ=21)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGc          >  1:1352724/1‑47 (MQ=21)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:375347/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:573412/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:571701/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:571151/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:527816/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:51753/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:511398/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:50962/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:489483/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:475893/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:452781/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:434002/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:586392/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:335110/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:324842/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:304001/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:295219/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:293316/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:276571/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:25355/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:221400/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:10139/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:785236/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:982869/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:981718/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:938366/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:921018/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:894844/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:877902/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:864880/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:860555/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:829455/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:810573/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:785805/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:585723/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:78491/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:761771/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:749873/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:724258/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:70284/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:690128/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:687431/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:671177/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:659935/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:587731/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:200431/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1135087/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1270448/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1267425/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1265256/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1245131/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1225709/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1198822/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1197039/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1193063/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1185586/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1171038/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1170507/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:116780/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1305161/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1133166/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1111230/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1096948/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1089140/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1076639/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1074132/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1044149/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1035467/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:102740/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1026691/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1022711/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1447360/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1638675/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:160743/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1540790/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:15380/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1526779/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1526621/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1502202/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1486758/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1460760/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1454403/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1448306/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:196032/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:144167/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1438125/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1436134/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1431310/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1392575/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1363019/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1359664/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1354987/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1323222/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACgg  >  1:1313335/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGGCAAGACTACGCACTTCACCCGCCACCACgg  >  1:857342/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCGACTGCCAAGATTACGCACTTCACCCGCCACCACgg  >  1:477170/1‑55 (MQ=25)
tttGCCGCCTGTGCACTGCCACTGGCAAGATTACGCACTTCACCCg           >  1:456451/1‑46 (MQ=21)
           |                                           
TTTGCCGCCTGGGCGCTGCGACTGGCAAGATTACGCACTTCACCCGCCACCACGG  >  W3110S.gb/1973168‑1973222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑