Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,075,473 A→C K600Q (AAA→CAG)  flu → antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,075,4730AC100.0% 209.2 / NA 72K600Q (AAA→CAG) fluantigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/72);  total (0/72)

GCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCC  >  W3110S.gb/2075464‑2075524
         |                                                   
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATTGCACCATCAGCCTGCGTGTACGcc  <  1:1149448/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:382611/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:514092/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:511968/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:48960/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:489158/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:467313/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:466941/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:464712/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:427572/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:421840/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:520779/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:378870/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:32039/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:317611/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:300124/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:295648/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:269638/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:210937/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:692076/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:931595/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:913603/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:843815/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:798134/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:781514/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:774453/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:742650/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:693507/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:204557/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:62282/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:603751/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:586219/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:581928/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:560609/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:554765/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:547274/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1203385/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1374325/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1368475/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1325772/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:129404/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1292876/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1286005/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1283945/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1267250/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1395637/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1195800/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1136931/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1094657/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1084413/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1056902/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1047772/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:104390/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1042794/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1595575/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:199783/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:195568/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:176634/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:169245/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1643149/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1632118/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1602427/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1009948/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1573549/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1535621/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1503670/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1492442/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1479993/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:146047/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:1450983/60‑1 (MQ=255)
acAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACAATCAGCCTGCGTGTACGcc  <  1:1460759/60‑1 (MQ=255)
 cAACTCTGCAGGTGAAAAACCTGAACGGGCAGAATGGCACCATCAGCCTGCGTGTACGcc  <  1:239165/60‑1 (MQ=255)
         |                                                   
GCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCC  >  W3110S.gb/2075464‑2075524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑