| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,075,889 | T→C | N738N (AAT→AAC) | flu → | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,075,889 | 0 | T | C | 97.1% | 284.3 / ‑2.8 | 104 | N738N (AAT→AAC) | flu | antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (101/0); total (104/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATTGTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAATGGTGAAAACAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAT > W3110S.gb/2075851‑2075943 | aTTGTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAATGGTGAAaac > 1:1125111/1‑48 (MQ=255)aTTGTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAATGGTGAAaac > 1:1516410/1‑48 (MQ=255)aTTGTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAATGGTGAAaac > 1:359379/1‑48 (MQ=255) tgtAAACGGTGAAAGTAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1572420/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTc > 1:1323295/1‑38 (MQ=38) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCgg > 1:1444742/1‑45 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCAtctc > 1:891718/1‑52 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCAtct > 1:373886/1‑51 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCAtc > 1:1491865/1‑50 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:314414/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:475635/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:441266/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:43276/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:413111/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:402446/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:382839/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:347201/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:544279/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:288782/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:281118/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:280532/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:25944/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:235616/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:232494/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:222561/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:207757/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:185627/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:177583/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:754848/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:969385/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:949500/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:900378/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:884433/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:878865/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:874111/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:844944/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:833089/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:818959/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:802613/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:793385/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:773309/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:476377/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:745885/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:739587/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:732698/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:712029/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:706351/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:697892/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:697123/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:660869/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:591165/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1624160/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:529738/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1204205/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1383530/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1335984/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1335021/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1333017/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1330532/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1316812/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1285826/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1270911/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1224851/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1223302/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:121700/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1395863/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1175094/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:117423/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1147302/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1136157/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1122334/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1116271/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1106209/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:107282/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1031465/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1022742/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1015277/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1555388/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1650182/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1625191/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1010170/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1611949/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1592902/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1591192/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:157654/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1570600/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1568650/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1564704/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1562797/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:177008/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:155069/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1546397/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1523902/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1487573/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1480897/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1459783/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1443041/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1432532/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1422050/1‑61 (MQ=255) tgtAAACGGTGAAAATAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAt > 1:1407939/1‑61 (MQ=255) | ATTGTGGCAGGCTCCCGCAGCCATCAGACCGGTGTAAATGGTGAAAACAACAGCGTCCGTCTCAGCATTCAGGGCGGTCATCTCGGTCACGAT > W3110S.gb/2075851‑2075943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |