Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,084,409 G→A R92R (CGC→CGT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,084,4090GA100.0% 424.2 / NA 148R92R (CGC→CGT) dacDD‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (148/0);  total (148/0)

Only 100 of 148 total aligned reads displayed.

AAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACG  >  W3110S.gb/2084357‑2084418
                                                    |         
aaaaaaTCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:438208/1‑62 (MQ=255)
aaaaCATCAGTGAAGGACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1062380/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGccc      >  1:1213819/1‑58 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCAc   >  1:819642/1‑61 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCAc   >  1:501537/1‑61 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCAc   >  1:821554/1‑61 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCAc   >  1:1101159/1‑61 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:462238/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:430160/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:441896/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:443689/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:405910/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:519785/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:531204/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:533567/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:557261/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:569576/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:428705/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:41388/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:591380/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:351400/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:347102/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:330611/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:318496/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:297409/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:283118/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:250940/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:248028/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:241752/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:731245/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:978873/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:96510/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:949930/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:90385/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:886876/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:87687/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:849526/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:836002/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:781032/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:769358/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:73729/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:572862/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:684916/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:682809/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:653419/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:649866/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:626929/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:625377/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:602133/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:60181/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:236049/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:585369/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1143051/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1298192/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1267388/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1257861/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1236234/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1219007/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:121665/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1214360/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:118972/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1188782/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1168667/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1145777/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1298666/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1126971/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1107681/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:110663/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1103568/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1083186/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:107402/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1064732/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1056870/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1047115/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1029246/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1015310/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1484607/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1007671/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:213418/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:211903/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:20126/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:192729/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1625844/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1617583/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1583723/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1565468/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1517942/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1505458/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:236554/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1437700/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1407622/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1405702/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1382495/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1371297/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1348680/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1340983/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1329767/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1328289/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1323310/1‑62 (MQ=255)
aaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACg  >  1:1317495/1‑62 (MQ=255)
                                                    |         
AAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACG  >  W3110S.gb/2084357‑2084418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑